Leukotriene B4 Receptor/BLT Antikörper (Biotin)

Produktnummer ABIN2688854

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Leukotriene B4 Receptor/BLT in FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Leukotriene B4 Receptor/BLT Antikörper (Biotin) (ABIN2688854)

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Leukotriene B4 Receptor/BLT Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: 203-14F11

Produktdetails anti-Leukotriene B4 Receptor/BLT Antikörper (Biotin)

Marke: BD Pharmingen™

Produktmerkmale: The monoclonal antibody 203/14F11 reacts against human leukotriene B4 receptor 1 (BLTR-1). BLTR-1 is a seven-transmembrane, G-protein-coupled receptor. The receptor plays an important role in pro-inflammatory responses. Leukotrienne B4 receptors are distributed in various hematopoietic cells including monocytes, granulocytes, and lymphocytes. Leukotriene B4 (LTB4), a product of arachidonic acid metabolism, is the ligand for BLTR-1. The immunogen used to generate 203/14F11 hybridoma was a human BLTR-1-transfected cell line. Detection of BLTR-1 expression on human peripheral monocytes and granulocytes by biotinylated 203/14F11. Human lysed whole blood were stained with 20 μL/test of biotinylated 203/14F11. Streptavidin-PE (Cat. No. 554061) was used as second-step reagent. The data reflects gating on monocytes (left panel) and granulocytes (right panel) based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated mouse IgG1 (Cat. No. 555747) as isotype control (shaded histogram).

BD Pharmingen™ Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor) - Biotin - Clone 203/14F11 - Isotype Mouse IgG1 - Reactivity Hu - 100 Tests

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human BLTR-1-transfected cell line

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-Leukotriene B4 Receptor/BLT Antikörper (Biotin) (ABIN2688854)

Pettersson, Boketoft, Sabirsh, Nilsson, Kotarsky, Olde, Owman: "First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 520-5, (2001) (PubMed).

Dasari, Jin, Kunapuli: "Distribution of leukotriene B4 receptors in human hematopoietic cells." in: Immunopharmacology, Vol. 48, Issue 2, pp. 157-63, (2000) (PubMed).

Yokomizo, Masuda, Kato, Toda, Izumi, Shimizu: "Leukotriene B4 receptor. Cloning and intracellular signaling." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 2 Pt 2, pp. S51-5, (2000) (PubMed).

Details zu Leukotriene B4 Receptor/BLT

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Andere Bezeichnung: BLTR-1Leukotriene B4 Receptor

Hintergrund: Synonyms: hBLTR-1