14-3-3 theta Antikörper

Produktnummer ABIN2688837

Der Maus Monoklonal Anti-14-3-3 theta-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, 14-3-3 theta in Proben von Human, Maus, Ratte, Huhn, Hund und Frosch zu detektieren.

Kurzübersicht für 14-3-3 theta Antikörper (ABIN2688837)

Target: 14-3-3 theta (YWHAQ)

Reaktivität: Human, Maus, Ratte, Huhn, Hund, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser 14-3-3 theta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 12-14-3

Produktdetails anti-14-3-3 theta Antikörper

Produktmerkmale: The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ. Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti- 14-3-3 antibody. Immunofluorescence staining of SKN cells (Human neuroblastoma).

BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3 - Purified - Clone 12/14-3-3 - Isotype Mouse IgM - Reactivity Hu, Chick, Dog, Frog, Ms, Rat - 50 μg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human 14-3-3

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Details zu 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Andere Bezeichnung: 14-3-3

Molekulargewicht: 29 kDa

Pathways: Apoptose, Myometrial Relaxation and Contraction