SP1 Antikörper (pSer101)

Produktnummer ABIN2668960

Der Kaninchen Polyklonal anti-SP1 Antikörper wird verwendet zum Nachweis von SP1 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für SP1 Antikörper (pSer101) (ABIN2668960)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SP1 Antikörper

Bindungsspezifität: pSer101

Verwendungszweck: Sp1 phospho Ser101 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This Sp1 phospho Ser101 antibody was raised against a peptide containing phospho-serine 101 of human Sp1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Andere Bezeichnung: Sp1

Hintergrund: Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.

Molekulargewicht: 96 kDa

Gen-ID: 6667

NCBI Accession: NP_612482

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction