ASH2L Antikörper

Produktnummer ABIN2668850

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-ASH2L Antikörper (ABIN2668850) detektiert spezifisch ASH2L in WB, ChIP und ChIP-seq. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ASH2L Antikörper (ABIN2668850)

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASH2L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-ASH2L Antikörper

Verwendungszweck: ASH2L antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This ASH2L antibody was raised against full-length recombinant human ASH2L protein.

Anwendungsinformationen

Applikationshinweise: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB*: 1:500 - 1:2,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-ASH2L Antikörper (ABIN2668850)

Fossati, Dolfini, Donati, Mantovani: "NF-Y recruits Ash2L to impart H3K4 trimethylation on CCAAT promoters." in: PLoS ONE, Vol. 6, Issue 3, pp. e17220, (2011) (PubMed).

Cao, Chen, Cierpicki, Liu, Basrur, Lei, Dou: "An Ash2L/RbBP5 heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1 SET domain." in: PLoS ONE, Vol. 5, Issue 11, pp. e14102, (2010) (PubMed).

Details zu ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Andere Bezeichnung: ASH2L

Hintergrund: ASH2L (absent, small or homeotic discs 2) is a trithorax protein and is a component of different protein complexes, including i.e. mixed-lineage leukemia (MLL) oncoproteins, which contain histone methyltransferase activity. These complexes trimethylate the lysine 4 of histone H3. Histone H3 trimethyl Lys4 is one of histone modifications that are part of elaborate mechanisms that evolved to control the structure of the chromatin.

Molekulargewicht: 77 kDa

Gen-ID: 430

NCBI Accession: NP_004665