Lamin A/C Antikörper (AA 430-545)

Produktnummer ABIN7938325

Zitiert in 3+ Publikationen. Der Maus Monoklonal anti-Lamin A/C Antikörper (Klon 3A6-4C11) (ABIN7938325) detektiert spezifisch Lamin A/C in WB, IF, ICC, IP und ChIP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Affe und Hamster.

Kurzübersicht für Lamin A/C Antikörper (AA 430-545) (ABIN7938325)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Maus, Ratte, Affe, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Klon: 3A6-4C11

Produktdetails anti-Lamin A/C Antikörper

Bindungsspezifität: AA 430-545

Verwendungszweck: Lamin A/C antibody (mAb) (Clone 3A6-4C11)

Aufreinigung: Culture Supernatant

Immunogen: This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1:500 - 1:4000 dilution ICC/IF: 1:500 - 1:2000 dilution Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. For immunofluorescence experiments we recommend using paraformaldehyde (PFA) fixation. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91189.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Cell culture supernatant containing mouse IgG, 30 % glycerol, 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Lamin A/C Antikörper (ABIN7938325)

Gesson, Rescheneder, Skoruppa, von Haeseler, Dechat, Foisner: "A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2 alpha." in: Genome research, Vol. 26, Issue 4, pp. 462-73, (2016) (PubMed).

Kim, Zheng: "Generation and characterization of a conditional deletion allele for Lmna in mice." in: Biochemical and biophysical research communications, Vol. 440, Issue 1, pp. 8-13, (2013) (PubMed).

Eckersley-Maslin, Bergmann, Lazar, Spector: "Lamin A/C is expressed in pluripotent mouse embryonic stem cells." in: Nucleus (Austin, Tex.), Vol. 4, Issue 1, pp. 53-60, (2013) (PubMed).

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin A/C

Hintergrund: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Molekulargewicht: 75 kDa

Gen-ID: 4000

NCBI Accession: NP_733821

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus