Lamin A/C Antikörper (C-Term, Mature)

Produktnummer ABIN2668842

Dieser Maus Monoklonal Antikörper detektiert spezifisch Lamin A/C in WB, IF, ICC und IP. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Lamin A/C Antikörper (C-Term, Mature) (ABIN2668842)

Target: Lamin A/C (LMNA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 4A4

Produktdetails anti-Lamin A/C Antikörper

Bindungsspezifität: C-Term, Mature

Verwendungszweck: Lamin A, mature antibody (mAb) (Clone 4A4)

Aufreinigung: Protein G Chromatography

Immunogen: This Lamin A, mature antibody was raised against a peptide within the C-terminus of mature human Lamin A.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB*: 0.05 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Lamin A/C Antikörper (ABIN2668842)

Roblek, Schüchner, Huber, Ollram, Vlcek-Vesely, Foisner, Wehnert, Ogris: "Monoclonal antibodies specific for disease-associated point-mutants: lamin A/C R453W and R482W." in: PLoS ONE, Vol. 5, Issue 5, pp. e10604, (2010) (PubMed).

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin A

Hintergrund: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Molekulargewicht: 74 kDa

Gen-ID: 4000

NCBI Accession: NP_733821

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus