MITF Antikörper (N-Term)

Produktnummer ABIN2668714

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-MITF Antikörper wird verwendet zum Nachweis von MITF in Proben von Human. Er wurde validiert für WB, IF, ICC, IP, ChIP-seq, ChIP und SSA.

Kurzübersicht für MITF Antikörper (N-Term) (ABIN2668714)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Supershift Assay (SSA)

Klon: C5-D5

Produktdetails anti-MITF Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: MITF antibody (mAb) (Clone C5.D5)

Aufreinigung: Protein G Chromatography

Immunogen: This MITF antibody was raised against an N-terminal fragment of human MITF.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ChIP: 2.5 - 10 μg per ChIP ChIP-Seq: 4 μg each This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91201.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-MITF Antikörper (ABIN2668714)

Wellbrock, Marais: "Elevated expression of MITF counteracts B-RAF-stimulated melanocyte and melanoma cell proliferation." in: The Journal of cell biology, Vol. 170, Issue 5, pp. 703-8, (2005) (PubMed).

Du, Miller, Widlund, Horstmann, Ramaswamy, Fisher: "MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma." in: The American journal of pathology, Vol. 163, Issue 1, pp. 333-43, (2003) (PubMed).

Hemesath, Price, Takemoto, Badalian, Fisher: "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes." in: Nature, Vol. 391, Issue 6664, pp. 298-301, (1998) (PubMed).

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: MITF

Hintergrund: MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.

Molekulargewicht: 52-56 kDa

Gen-ID: 4286

NCBI Accession: NP_937802

Pathways: Chromatin Binding