RNF2 Antikörper

Produktnummer ABIN2668629

Dieser Maus Monoklonal Antikörper detektiert spezifisch RNF2 in WB, IF, IP, ChIP, ICC und ChIP-seq. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Affe. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für RNF2 Antikörper (ABIN2668629)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reaktivität: Human, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RNF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Klon: 3-3

Produktdetails anti-RNF2 Antikörper

Verwendungszweck: Ring1B antibody (mAb) (Clone 3-3)

Aufreinigung: Protein G Chromatography

Immunogen: This Ring1B antibody was raised against the recombinant full-length mouse protein.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 4 µL each

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-RNF2 Antikörper (ABIN2668629)

Jacinto, Benner, Hetzer: "The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing." in: Genes & development, Vol. 29, Issue 12, pp. 1224-38, (2015) (PubMed).

Basu, Wilkinson, Colavita, Fennelly, Atchison: "YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment." in: Nucleic acids research, Vol. 42, Issue 4, pp. 2208-23, (2014) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." in: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Lavial, Bessonnard, Ohnishi, Tsumura, Chandrashekran, Fenwick, Tomaz, Hosokawa, Nakayama, Chambers, Hiiragi, Chazaud, Azuara: "Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development." in: Genes & development, Vol. 26, Issue 13, pp. 1445-58, (2012) (PubMed).

Details zu RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Andere Bezeichnung: Ring1B

Hintergrund: Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.

Molekulargewicht: 40 kDa

Gen-ID: 6045

NCBI Accession: NP_009143