SIRT2 Antikörper (pSer331)

Produktnummer ABIN2668611

Dieser Ratte Monoklonal Antikörper detektiert spezifisch SIRT2 in WB. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für SIRT2 Antikörper (pSer331) (ABIN2668611)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SIRT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 6B5

Produktdetails anti-SIRT2 Antikörper

Bindungsspezifität: pSer331

Verwendungszweck: SIRT2 phospho Ser331 antibody (mAb) (Clone 6B5)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide corresponding to human SIRT2 phosphorylated at Serine 331.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-SIRT2 Antikörper (ABIN2668611)

Pandithage, Lilischkis, Harting, Wolf, Jedamzik, Lüscher-Firzlaff, Vervoorts, Lasonder, Kremmer, Knöll, Lüscher: "The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility." in: The Journal of cell biology, Vol. 180, Issue 5, pp. 915-29, (2008) (PubMed).

Details zu SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Andere Bezeichnung: SIRT2

Hintergrund: SIRT2 (sirtuin 2) is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARg and subsequent repression of PPARg mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis. Ser-331 is phosphorylated by the cyclin kinases Cdk2 and Cdk5 and this phosphorylation inhibits SIRT2's catalytic activity.

Molekulargewicht: 40 & 43 kDa

Gen-ID: 22933

NCBI Accession: NP_036369