CHD1 Antikörper (AA 251-467)

Produktnummer ABIN2668525

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-CHD1 Antikörper wird verwendet zum Nachweis von CHD1 in Proben von Human und Maus. Er wurde validiert für WB.

Kurzübersicht für CHD1 Antikörper (AA 251-467) (ABIN2668525)

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CHD1 Antikörper

Bindungsspezifität: AA 251-467

Verwendungszweck: CHD1 antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This CHD1 antibody was raised against a recombinant protein corresponding to amino acids 251-467 of human CHD1.

Anwendungsinformationen

Applikationshinweise: WB: 1:500 - 1:1,000 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-CHD1 Antikörper (ABIN2668525)

Skene, Hernandez, Groudine, Henikoff: "The nucleosomal barrier to promoter escape by RNA polymerase II is overcome by the chromatin remodeler Chd1." in: eLife, Vol. 3, pp. e02042, (2014) (PubMed).

Details zu CHD1

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Andere Bezeichnung: CHD1

Hintergrund: CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency.

Molekulargewicht: 220 kDa

Gen-ID: 1105

NCBI Accession: NP_001261

Pathways: Chromatin Binding