TWIST1 Antikörper

Produktnummer ABIN2668516

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-TWIST1 Antikörper wird verwendet zum Nachweis von TWIST1 in Proben von Human und Maus. Er wurde validiert für WB, IHC, ICC und IF.

Kurzübersicht für TWIST1 Antikörper (ABIN2668516)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TWIST1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-TWIST1 Antikörper

Verwendungszweck: TWIST antibody (mAb)

Aufreinigung: Protein G Chromatography

Immunogen: This TWIST antibody was raised against a recombinant protein corresponding to full-length mouse TWIST.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-TWIST1 Antikörper (ABIN2668516)

Ezponda, Popovic, Shah, Martinez-Garcia, Zheng, Min, Will, Neri, Kelleher, Yu, Licht: "The histone methyltransferase MMSET/WHSC1 activates TWIST1 to promote an epithelial-mesenchymal transition and invasive properties of prostate cancer." in: Oncogene, Vol. 32, Issue 23, pp. 2882-90, (2013) (PubMed).

Casas, Kim, Bendesky, Ohno-Machado, Wolfe, Yang: "Snail2 is an essential mediator of Twist1-induced epithelial mesenchymal transition and metastasis." in: Cancer research, Vol. 71, Issue 1, pp. 245-54, (2011) (PubMed).

Eckert, Lwin, Chang, Kim, Danis, Ohno-Machado, Yang: "Twist1-induced invadopodia formation promotes tumor metastasis." in: Cancer cell, Vol. 19, Issue 3, pp. 372-86, (2011) (PubMed).

Kida, Asahina, Teraoka, Gitelman, Sato: "Twist relates to tubular epithelial-mesenchymal transition and interstitial fibrogenesis in the obstructed kidney." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 55, Issue 7, pp. 661-73, (2007) (PubMed).

Gitelman: "Twist protein in mouse embryogenesis." in: Developmental biology, Vol. 189, Issue 2, pp. 205-14, (1997) (PubMed).

Details zu TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Andere Bezeichnung: TWIST

Hintergrund: TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial-mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.

Molekulargewicht: 21 kDa

Gen-ID: 7291

NCBI Accession: NP_000465

Pathways: p53 Signalweg, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity