Histone 3 Antikörper (H3K4me3)

Produktnummer ABIN2668474

Es sind 8+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Histone 3 Antikörper wird verwendet zum Nachweis von Histone 3 in Proben von Human und Saccharomyces cerevisiae. Er wurde validiert für WB, IF, ChIP, ICC, DB, ChIP-seq und CUT&RUN.

Kurzübersicht für Histone 3 Antikörper (H3K4me3) (ABIN2668474)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K4me3

Verwendungszweck: Histone H3K4me3 antibody (pAb)

Aufreinigung: Protein A Chromatography

Immunogen: This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 3 μg per ChIP ChIP-Seq: 4 μg each ICC/IF: 1 - 2 μg/mL dilution WB*: 2 - 4 μg/mL dilution CUT&Tag: 1 µL per 50 µL reaction* CUT&RUN: 1 µL per 50 µL reaction *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668474)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Akiyama, Koda, Byeon, Shimada, Nishikawaji, Sakamoto, Chen, Kojima, Kawano, Eishi, Deng, Kim, Zhu, Yuasa, Tanaka: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." in: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).

Singh, Johnstone, Martin, Tempera, Kaplan, Denny: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." in: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).

Heubach, Monsior, Deenen, Niegisch, Szarvas, Niedworok, Schulz, Hoffmann: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." in: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).

Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

Sakashita, Kawabata, Jincho, Tajima, Kumamoto, Kobayashi, Matsui, Kono: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).

Walter, Matter, Fahrenkrog: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).

DesRochers, Shamis, Alt-Holland, Kudo, Takata, Wang, Jackson-Grusby, Garlick: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." in: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522