Histone 3 Antikörper (H3K9me3)

Produktnummer ABIN2668470

Produktdetails anti-Histone 3 Antikörper

Target: Histone 3 (H3)

Bindungsspezifität: H3K9me3

Reaktivität: Human, Schizosaccharomyces pombe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Aufreinigung: None

Immunogen: This Histone H3 trimethylLys9 antibody was raised against a peptide including trimethyllysine 9 of histone H3.

Anwendungsinformationen

Applikationshinweise: Recommended antibody dilutions for different applications:
ChIP: 2 - 10 µl per ChIP
ChIP-Seq: 10 µl each
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:1,000 - 1:5,000 dilution
CUT&Tag: 1:50 dilution (1 µl per 50 µl reaction)
Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -20 °C

Informationen zur Lagerung: Antibodies in solution can be stored at -20 °C for 2 years.

Haltbarkeit: 6 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668470)

Egan, Yuan, Craske, Labhart, Guler, Arnott, Maile, Busby, Henry, Kelly, Tindell, Jhunjhunwala, Zhao, Hatton, Bryant, Classon, Trojer: "An Alternative Approach to ChIP-Seq Normalization Enables Detection of Genome-Wide Changes in Histone H3 Lysine 27 Trimethylation upon EZH2 Inhibition." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166438, (2016) (PubMed).

Thompson, Dulberg, Moon, Foster, Chen, Karimi, Lorincz: "hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells." in: PLoS genetics, Vol. 11, Issue 1, pp. e1004933, (2015) (PubMed).

Postberg, Kanders, Forcob, Willems, Orth, Hensel, Weil, Wirth, Jenke: "CpG signalling, H2A.Z/H3 acetylation and microRNA-mediated deferred self-attenuation orchestrate foetal NOS3 expression." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 9, (2015) (PubMed).

Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

Soyer, Möller, Schotanus, Connolly, Galazka, Freitag, Stukenbrock: "Chromatin analyses of Zymoseptoria tritici: Methods for chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq)." in: Fungal genetics and biology : FG & B, Vol. 79, pp. 63-70, (2015) (PubMed).

Amatori, Ballarini, Faversani, Belloni, Fusar, Bosari, Pelicci, Minucci, Fanelli: "PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples." in: Epigenetics & chromatin, Vol. 7, pp. 18, (2014) (PubMed).

Soyer, El Ghalid, Glaser, Ollivier, Linglin, Grandaubert, Balesdent, Connolly, Freitag, Rouxel, Fudal: "Epigenetic control of effector gene expression in the plant pathogenic fungus Leptosphaeria maculans." in: PLoS genetics, Vol. 10, Issue 3, pp. e1004227, (2014) (PubMed).

Minkovsky, Sahakyan, Rankin-Gee, Bonora, Patel, Plath: "The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation." in: Epigenetics & chromatin, Vol. 7, pp. 12, (2014) (PubMed).

Rangasamy: "Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons." in: Mobile DNA, Vol. 4, Issue 1, pp. 27, (2013) (PubMed).

Maksakova, Thompson, Goyal, Jones, Singh, Karimi, Lorincz: "Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific retrotransposon MERVL in mouse ES cells." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 15, (2013) (PubMed).

Thijssen, Tobi, Balog, Schouten, Kremer, El Bouazzaoui, Henneman, Putter, Eline Slagboom, Heijmans, van der Maarel: "Chromatin remodeling of human subtelomeres and TERRA promoters upon cellular senescence: commonalities and differences between chromosomes." in: Epigenetics, Vol. 8, Issue 5, pp. 512-21, (2013) (PubMed).

Karimi-Aghcheh, Bok, Phatale, Smith, Baker, Lichius, Omann, Zeilinger, Seiboth, Rhee, Keller, Freitag, Kubicek: "Functional analyses of Trichoderma reesei LAE1 reveal conserved and contrasting roles of this regulator." in: G3 (Bethesda, Md.), Vol. 3, Issue 2, pp. 369-78, (2013) (PubMed).

Connolly, Smith, Freitag: "The Fusarium graminearum histone H3 K27 methyltransferase KMT6 regulates development and expression of secondary metabolite gene clusters." in: PLoS genetics, Vol. 9, Issue 10, pp. e1003916, (2013) (PubMed).

Murata, Narita, Sugimoto, Kawashima, Kanda, Tsurumi: "Contribution of myocyte enhancer factor 2 family transcription factors to BZLF1 expression in Epstein-Barr virus reactivation from latency." in: Journal of virology, Vol. 87, Issue 18, pp. 10148-62, (2013) (PubMed).

Wiemann, Sieber, von Bargen, Studt, Niehaus, Espino, Huß, Michielse, Albermann, Wagner, Bergner, Connolly, Fischer, Reuter, Kleigrewe, Bald, Wingfield, Ophir, Freeman, Hippler, Smith, Brown, Proctor et al.: "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites. ..." in: PLoS pathogens, Vol. 9, Issue 6, pp. e1003475, (2013) (PubMed).

Jamieson, Rountree, Lewis, Stajich, Selker: "Regional control of histone H3 lysine 27 methylation in Neurospora." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 15, pp. 6027-32, (2013) (PubMed).

Murphy, Cipriany, Wallin, Ju, Szeto, Hagarman, Benitez, Craighead, Soloway: "Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7772-7, (2013) (PubMed).

Woellmer, Arteaga-Salas, Hammerschmidt: "BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression." in: PLoS pathogens, Vol. 8, Issue 9, pp. e1002902, (2012) (PubMed).

Seiboth, Karimi, Phatale, Linke, Hartl, Sauer, Smith, Baker, Freitag, Kubicek: "The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei." in: Molecular microbiology, Vol. 84, Issue 6, pp. 1150-64, (2012) (PubMed).

Duncan, Barwick, Jin, Rago, Kapoor-Vazirani, Powell, Chi, Bigner, Vertino, Yan: "A heterozygous IDH1R132H/WT mutation induces genome-wide alterations in DNA methylation." in: Genome research, Vol. 22, Issue 12, pp. 2339-55, (2012) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3 (H3 Produkte)

Molekulargewicht: 17 kDa

Gen-ID: 3020