Histone 3 Antikörper (H3K122me1)

Produktnummer ABIN2668462

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-Histone 3 Antikörper wird verwendet zum Nachweis von Histone 3 in Proben von Human. Er wurde validiert für WB und DB.

Kurzübersicht für Histone 3 Antikörper (H3K122me1) (ABIN2668462)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K122me1

Verwendungszweck: Histone H3K122me1 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This Histone H3 monomethyl Lys122 antibody was raised against a peptide containing monomethyl-lysine 122 of human histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:250 - 1:1,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668462)

Ivanauskiene, Delbarre, McGhie, Küntziger, Wong, Collas: "The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity." in: Genome research, Vol. 24, Issue 10, pp. 1584-94, (2014) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522