Histone 3 Antikörper (H3K36ac)

Produktnummer ABIN2668454

Zitiert in 3+ Publikationen. Der Kaninchen Polyklonal anti-Histone 3 Antikörper (ABIN2668454) detektiert spezifisch Histone 3 in WB, IF, ChIP, ICC, DB und ChIP-seq. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone 3 Antikörper (H3K36ac) (ABIN2668454)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K36ac

Verwendungszweck: Histone H3K36ac antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H3 acetyl Lys36 antibody was raised against a peptide including acetyl-lysine 36 of histone H3.

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 µL per ChIP ICC/IF: 1:500 - 1:2,000 dilution WB: 1:500 - 1:2,000 dilution The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 acetyl Lys36 antibody was validated for ChIP-Seq in the study (see reference).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668454)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Gacias, Gerona-Navarro, Plotnikov, Zhang, Zeng, Kaur, Moy, Rusinova, Rodriguez, Matikainen, Vincek, Joshua, Casaccia, Zhou: "Selective chemical modulation of gene transcription favors oligodendrocyte lineage progression." in: Chemistry & biology, Vol. 21, Issue 7, pp. 841-854, (2014) (PubMed).

Egelhofer, Minoda, Klugman, Lee, Kolasinska-Zwierz, Alekseyenko, Cheung, Day, Gadel, Gorchakov, Gu, Kharchenko, Kuan, Latorre, Linder-Basso, Luu, Ngo, Perry, Rechtsteiner, Riddle, Schwartz, Shanower et al.: "An assessment of histone-modification antibody quality. ..." in: Nature structural & molecular biology, Vol. 18, Issue 1, pp. 91-3, (2011) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Histone H3 Lys36 can also be mono-, di- or trimethylated. Methylation of Histone H3 Lys36 is associated with transcriptional repression.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522