Histone 3 Antikörper (H3K4ac)

Produktnummer ABIN2668453

Es sind 6+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Histone 3 Antikörper wird verwendet zum Nachweis von Histone 3 in Proben von Human. Er wurde validiert für WB, IF, ChIP, ICC, DB und ChIP-seq.

Kurzübersicht für Histone 3 Antikörper (H3K4ac) (ABIN2668453)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K4ac

Verwendungszweck: Histone H3K4ac antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H3 acetyl Lys4 antibody was raised against a peptide containing acetyl-lysine 4 of histone H3.

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 µL per ChIP ChIP-Seq: 5 - 10 µL each ICC/IF: 1:500 - 1:2,000 dilution WB: 1:1,000 - 1:2,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668453)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Messier, Gordon, Boyd, Tye, Browne, Stein, Lian, Stein: "Histone H3 lysine 4 acetylation and methylation dynamics define breast cancer subtypes." in: Oncotarget, Vol. 7, Issue 5, pp. 5094-109, (2016) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Hagelkruys, Lagger, Krahmer, Leopoldi, Artaker, Pusch, Zezula, Weissmann, Xie, Schöfer, Schlederer, Brosch, Matthias, Selfridge, Lassmann, Knoblich, Seiser: "A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain development in the absence of its paralog." in: Development (Cambridge, England), Vol. 141, Issue 3, pp. 604-16, (2014) (PubMed).

Zhang, Lei, Lu: "Alterations of epigenetic signatures in hepatocyte nuclear factor 4? deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays." in: PLoS ONE, Vol. 9, Issue 1, pp. e84925, (2014) (PubMed).

Ma, Pan, Montgomery, Olson, Schultz: "Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 8, pp. E481-9, (2012) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Lysine 4 of histone H3 can also be mono-, di- or trimethylated. This methylation is associated with transcriptional activation.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522