Histone 3 Antikörper (H3R8me2a)

Produktnummer ABIN2668406

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-Histone 3 Antikörper (ABIN2668406) detektiert spezifisch Histone 3 in WB, ChIP, DB und ChIP-seq. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Histone 3 Antikörper (H3R8me2a) (ABIN2668406)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3R8me2a

Verwendungszweck: Histone H3R8me2a (asymmetric) antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB: 1:500 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668406)

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." in: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Blythe, Cha, Tadjuidje, Heasman, Klein: "beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2." in: Developmental cell, Vol. 19, Issue 2, pp. 220-31, (2010) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522