Histone 3 Antikörper (H3K36me2)

Produktnummer ABIN2668402

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-Histone 3 Antikörper wird verwendet zum Nachweis von Histone 3 in Proben von Human. Er wurde validiert für WB, IF, ChIP und DB.

Kurzübersicht für Histone 3 Antikörper (H3K36me2) (ABIN2668402)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Klon: MABI 0332

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K36me2

Verwendungszweck: Histone H3K36me2 antibody (mAb) (Clone MABI 0332)

Aufreinigung: Protein G Chromatography

Immunogen: This Histone H3 dimethyl Lys36 antibody was raised against a peptide containing dimethyl Lys36 of human Histone H3.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 0.5 - 2 μg/mL dilution DB: 0.5 - 2 μg/mL dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.69 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668402)

Amendola, Zaghet, Ramalho, Vilstrup Johansen, Boxem, Salcini: "JMJD-5/KDM8 regulates H3K36me2 and is required for late steps of homologous recombination and genome integrity." in: PLoS genetics, Vol. 13, Issue 2, pp. e1006632, (2017) (PubMed).

García-Carpizo, Sarmentero, Han, Graña, Ruiz-Llorente, Pisano, Serrano, Brooks, Campbell, Barrero: "NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation." in: Scientific reports, Vol. 6, pp. 32952, (2016) (PubMed).

Rechtsteiner, Ercan, Takasaki, Phippen, Egelhofer, Wang, Kimura, Lieb, Strome: "The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny." in: PLoS genetics, Vol. 6, Issue 9, pp. e1001091, (2010) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522