Histone 3 Antikörper (H3K9me1)

Produktnummer ABIN2668394

Zitiert in 2+ Publikationen. Der Maus Monoklonal anti-Histone 3 Antikörper (Klon MABI 0306) (ABIN2668394) detektiert spezifisch Histone 3 in WB, ChIP und DB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone 3 Antikörper (H3K9me1) (ABIN2668394)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Klon: MABI 0306

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K9me1

Verwendungszweck: Histone H3K9me1 antibody (mAb) (Clone MABI 0306)

Aufreinigung: Protein G Chromatography

Immunogen: This Histone H3 monomethyl Lys9 antibody was raised against a synthetic peptide containing monomethyl Lys9 of human histone H3.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 0.5 - 2 μg/mL dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.4 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668394)

Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

Gregory, Cheung: "Natural variation in the histone demethylase, KDM4C, influences expression levels of specific genes including those that affect cell growth." in: Genome research, Vol. 24, Issue 1, pp. 52-63, (2014) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522