Histone 3 Antikörper (H3K4me2)

Produktnummer ABIN2668392

Dieser Maus Monoklonal Antikörper detektiert spezifisch Histone 3 in WB, IF, ChIP, ICC, DB und ChIP-seq. Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für Histone 3 Antikörper (H3K4me2) (ABIN2668392)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Klon: MABI 0303

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K4me2

Verwendungszweck: Histone H3K4me2 antibody (mAb) (Clone MABI 0303)

Aufreinigung: Protein G Chromatography

Immunogen: This Histone H3 dimethyl Lys4 antibody was raised against a synthetic peptide containing dimethyl Lys4 of human histone H3.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 1 - 2 µL/mL dilution WB: 2 - 5 μg/mL dilution DB: 1 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For Histone H3K4me2, we also offer AbFlex Histone H3K4me2 Recombinant Antibody (rAb). For details, see Catalog No. 91321.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.46 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol, 0.3 M NaCl, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668392)

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." in: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522