Histone 3 Antikörper (N-Term)

Produktnummer ABIN2668390

Es sind 9+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-Histone 3 Antikörper wird verwendet zum Nachweis von Histone 3 in Proben von Human. Er wurde validiert für IF, ChIP, ICC und ChIP-seq.

Kurzübersicht für Histone 3 Antikörper (N-Term) (ABIN2668390)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Klon: MABI 0301

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Histone H3 antibody (mAb) (Clone MABI 0301)

Aufreinigung: Protein G Chromatography

Immunogen: This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 4 μg per ChIP ChIP: 5 - 10 μg per ChIP ICC/IF: 1 μg/mL dilution For Histone H3, we also offer AbFlex Histone H3 Recombinant Antibody (rAb). For details, see Catalog No. 91297.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.54 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668390)

Bulusu, Tumanov, Michalopoulou, van den Broek, MacKay, Nixon, Dhayade, Schug, Vande Voorde, Blyth, Gottlieb, Vazquez, Kamphorst: "Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation." in: Cell reports, Vol. 18, Issue 3, pp. 647-658, (2017) (PubMed).

Pacaud, Cheray, Nadaradjane, Vallette, Cartron: "Histone H3 phosphorylation in GBM: a new rational to guide the use of kinase inhibitors in anti-GBM therapy." in: Theranostics, Vol. 5, Issue 1, pp. 12-22, (2015) (PubMed).

Wagner, Ciszewski, Kania: "L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation." in: Cell communication and signaling : CCS, Vol. 13, pp. 36, (2015) (PubMed).

Zhan, Kost-Alimova, Shi, Leo, Bardenhagen, Shepard, Appikonda, Vangamudi, Zhao, Tieu, Jiang, Heffernan, Marszalek, Toniatti, Draetta, Tyler, Barton, Jones, Palmer, Geck Do, Andersen: "Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery." in: Epigenetics & chromatin, Vol. 8, pp. 37, (2015) (PubMed).

Fukuda, Mitani, Miyashita, Umezawa, Akutsu: "Chromatin condensation of Xist genomic loci during oogenesis in mice." in: Development (Cambridge, England), Vol. 142, Issue 23, pp. 4049-55, (2015) (PubMed).

van de Werken, van der Heijden, Eleveld, Teeuwssen, Albert, Baarends, Laven, Peters, Baart: "Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications." in: Nature communications, Vol. 5, pp. 5868, (2014) (PubMed).

Murphy, Cipriany, Wallin, Ju, Szeto, Hagarman, Benitez, Craighead, Soloway: "Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7772-7, (2013) (PubMed).

Ohmori, Takai, Ishijima, Suzuki, Moriguchi, Philipsen, Yamamoto, Ohneda: "Regulation of GATA factor expression is distinct between erythroid and mast cell lineages." in: Molecular and cellular biology, Vol. 32, Issue 23, pp. 4742-55, (2012) (PubMed).

Cerf, Tian, Craighead: "Ordered arrays of native chromatin molecules for high-resolution imaging and analysis." in: ACS nano, Vol. 6, Issue 9, pp. 7928-34, (2012) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522