Histone 3 Antikörper (H3K9me3)

Produktnummer ABIN2668380

Dieser Maus Monoklonal Antikörper detektiert spezifisch Histone 3 in WB, IF, ICC und DB. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für Histone 3 Antikörper (H3K9me3) (ABIN2668380)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Klon: 2AG-6F12-H4

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K9me3

Verwendungszweck: Histone H3K9me3 antibody (mAb) (Clone 2AG-6F12-H4)

Aufreinigung: Culture Supernatant

Immunogen: This Histone H3 trimethyl Lys9 antibody was raised against a peptide containing trimethyl-lysine 9 of human histone H3.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ICC/IF: 1:20 - 1:100 dilution WB*: 1:500 - 1:5,000 dilution DB: 1:5,000 dilution This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: Catalog No. 39161. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Cell culture supernatant containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone 3 Antikörper (ABIN2668380)

Carmona-Mora, Widagdo, Tomasetig, Canales, Cha, Lee, Alshawaf, Dottori, Whan, Hardeman, Palmer: "The nuclear localization pattern and interaction partners of GTF2IRD1 demonstrate a role in chromatin regulation." in: Human genetics, Vol. 134, Issue 10, pp. 1099-115, (2015) (PubMed).

Hübner, Lomiento, Mammoli, Illner, Markaki, Ferrari, Cremer, Cremer: "Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments." in: Epigenetics & chromatin, Vol. 8, pp. 47, (2015) (PubMed).

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." in: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Boyarchuk, Filipescu, Vassias, Cantaloube, Almouzni: "The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle." in: Journal of cell science, Vol. 127, Issue Pt 15, pp. 3347-59, (2014) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Molekulargewicht: 17 kDa

Gen-ID: 3020

NCBI Accession: NP_003522