CENPA Antikörper (pSer18)

Produktnummer ABIN2668362

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CENPA in WB, IF, ICC und DB. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für CENPA Antikörper (pSer18) (ABIN2668362)

Target: CENPA (Centromere Protein A (CENPA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CENPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Produktdetails anti-CENPA Antikörper

Bindungsspezifität: pSer18

Verwendungszweck: CENP-A phospho Ser18 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ICC/IF: 1:100 dilution WB: 1:100 - 1:500 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-CENPA Antikörper (ABIN2668362)

Logsdon, Barrey, Bassett, DeNizio, Guo, Panchenko, Dawicki-McKenna, Heun, Black: "Both tails and the centromere targeting domain of CENP-A are required for centromere establishment." in: The Journal of cell biology, Vol. 208, Issue 5, pp. 521-31, (2015) (PubMed).

Details zu CENPA

Target: CENPA (Centromere Protein A (CENPA))

Andere Bezeichnung: CENP-A

Hintergrund: CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis.

Molekulargewicht: 16 kDa

Gen-ID: 1058

NCBI Accession: NP_001800

Pathways: Chromatin Binding, Maintenance of Protein Location