HDAC4 Antikörper (AA 194-209)

Produktnummer ABIN2668313

Zitiert in 9+ Publikationen. Der Kaninchen Polyklonal anti-HDAC4 Antikörper (ABIN2668313) detektiert spezifisch HDAC4 in WB, ChIP und ChIP-seq. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für HDAC4 Antikörper (AA 194-209) (ABIN2668313)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-HDAC4 Antikörper

Bindungsspezifität: AA 194-209

Verwendungszweck: HDAC4 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This HDAC4 antibody was raised against a synthetic peptide corresponding to amino acid residues 194-209 of human HDAC4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each WB: 1 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 μg/μL

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-HDAC4 Antikörper (ABIN2668313)

Liu, Gu, Feng, Yang, Zhu, Lu, Qi: "Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells." in: Journal of translational medicine, Vol. 14, pp. 7, (2016) (PubMed).

Isaacs, Antony, Dalrymple, Brennen, Gerber, Hammers, Wissing, Kachhap, Luo, Xing, Björk, Olsson, Björk, Leanderson: "Tasquinimod Is an Allosteric Modulator of HDAC4 survival signaling within the compromised cancer microenvironment." in: Cancer research, Vol. 73, Issue 4, pp. 1386-99, (2013) (PubMed).

Keedy, Archin, Gates, Espeseth, Hazuda, Margolis: "A limited group of class I histone deacetylases acts to repress human immunodeficiency virus type 1 expression." in: Journal of virology, Vol. 83, Issue 10, pp. 4749-56, (2009) (PubMed).

Mottet, Bellahcène, Pirotte, Waltregny, Deroanne, Lamour, Lidereau, Castronovo: "Histone deacetylase 7 silencing alters endothelial cell migration, a key step in angiogenesis." in: Circulation research, Vol. 101, Issue 12, pp. 1237-46, (2007) (PubMed).

Sayeed, Konduri, Liu, Bansal, Li, Das: "Estrogen receptor alpha inhibits p53-mediated transcriptional repression: implications for the regulation of apoptosis." in: Cancer research, Vol. 67, Issue 16, pp. 7746-55, (2007) (PubMed).

Testoni, Mantovani: "Mechanisms of transcriptional repression of cell-cycle G2/M promoters by p63." in: Nucleic acids research, Vol. 34, Issue 3, pp. 928-38, (2006) (PubMed).

Basile, Mantovani, Imbriano: "DNA damage promotes histone deacetylase 4 nuclear localization and repression of G2/M promoters, via p53 C-terminal lysines." in: The Journal of biological chemistry, Vol. 281, Issue 4, pp. 2347-57, (2006) (PubMed).

Imbriano, Gurtner, Cocchiarella, Di Agostino, Basile, Gostissa, Dobbelstein, Del Sal, Piaggio, Mantovani: "Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters." in: Molecular and cellular biology, Vol. 25, Issue 9, pp. 3737-51, (2005) (PubMed).

Caretti, Salsi, Vecchi, Imbriano, Mantovani: "Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 30435-40, (2003) (PubMed).

Details zu HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Andere Bezeichnung: HDAC4

Hintergrund: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

Molekulargewicht: 140 kDa

Gen-ID: 9759

NCBI Accession: NP_006028

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process