Histone H2A Antikörper (H2AS129ph)

Produktnummer ABIN2668303

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-Histone H2A Antikörper wird verwendet zum Nachweis von Histone H2A in Proben von Saccharomyces cerevisiae. Er wurde validiert für WB, DB und ChIP.

Kurzübersicht für Histone H2A Antikörper (H2AS129ph) (ABIN2668303)

Target: Histone H2A

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: H2AS129ph

Verwendungszweck: Histone H2AS129ph antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H2A phospho Ser129 antibody was raised against a peptide including phospho-serine 129 of budding yeast Histone H2A.

Anwendungsinformationen

Applikationshinweise: WB: 1:500 - 1:2,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H2A Antikörper (ABIN2668303)

Jha, Strahl: "An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair." in: Nature communications, Vol. 5, pp. 3965, (2014) (PubMed).

Details zu Histone H2A

Target: Histone H2A

Hintergrund: Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2A variant, H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2A at serine 129 is required for the efficient repair of DNA double-strand breaks.

Molekulargewicht: 14 kDa

NCBI Accession: NP_010511