KDM4A Antikörper (AA 586-674)

Produktnummer ABIN2668289

Der Maus Monoklonal anti-KDM4A Antikörper (Klon N154-32) (ABIN2668289) detektiert spezifisch KDM4A in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für KDM4A Antikörper (AA 586-674) (ABIN2668289)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KDM4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: N154-32

Produktdetails anti-KDM4A Antikörper

Bindungsspezifität: AA 586-674

Verwendungszweck: JMJD2A antibody (mAb) (Clone N154/32)

Aufreinigung: Protein A Chromatography

Immunogen: This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB*: 1 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91143.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu KDM4A

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Andere Bezeichnung: JMJD2A

Hintergrund: JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

Molekulargewicht: 130 kDa

Gen-ID: 9682

NCBI Accession: NP_055478

Pathways: Warburg Effekt