Notch1 Antikörper

Produktnummer ABIN2668271

Der Ratte Monoklonal anti-Notch1 Antikörper (Klon ICN 5B5) (ABIN2668271) detektiert spezifisch Notch1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für Notch1 Antikörper (ABIN2668271)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Human, Ratte

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Notch1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: ICN 5B5

Produktdetails anti-Notch1 Antikörper

Verwendungszweck: Notch1 antibody (mAb) (Clone ICN 5B5)

Aufreinigung: Protein G Chromatography

Immunogen: This Notch1 antibody was raised against a recombinant protein corresponding to the intracellular domain of human Notch1.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 - 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91243.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: Notch1

Hintergrund: Notch1 is a member of the Notch family of receptors, which make up a highly conserved cell signaling system. Notch1 functions as a ligand-activated transcription factor that directly transduces extracellular signals in the cell surface into changes in gene expression in the nucleus. Notch1 is membrane bound, serving as an extracellular receptor for the Delta and Serrate family of ligands. Upon activation, the intracellular domain is cleaved and rapidly translocates into the nucleus where it forms a transcriptional complex with the RBP-J DNA binding protein and activates gene expression via recruitment of co-activators of the MAML family. Notch receptors play a critical role in cell fate specification during development and participate in multiple biological processes. Notch signaling is required for undifferentiated embryonic stem cells (ESCs) to participate in the formation of all three embryonic germ layers.

Molekulargewicht: 120 kDa

Gen-ID: 4851

NCBI Accession: NP_060087

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development