DNMT3B Antikörper (AA 1-218)

Produktnummer ABIN2668253

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-DNMT3B Antikörper (ABIN2668253) detektiert spezifisch DNMT3B in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für DNMT3B Antikörper (AA 1-218) (ABIN2668253)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNMT3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DNMT3B Antikörper

Bindungsspezifität: AA 1-218

Verwendungszweck: DNMT3B antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This DNMT3B antibody was raised against a recombinant protein corresponding to amino acids 1-218 of mouse DNMT3B.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-DNMT3B Antikörper (ABIN2668253)

Ajj, Chesnel, Pinel, Plenat, Flament, Dumond: "An alkylphenol mix promotes seminoma derived cell proliferation through an ERalpha36-mediated mechanism." in: PLoS ONE, Vol. 8, Issue 4, pp. e61758, (2013) (PubMed).

Details zu DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Andere Bezeichnung: DNMT3B

Hintergrund: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.

Molekulargewicht: 110 kDa

Gen-ID: 13436

NCBI Accession: NP_001003960