AGO1 Antikörper (N-Term)

Produktnummer ABIN2668226

Zitiert in 2+ Publikationen. Der Ratte Monoklonal anti-AGO1 Antikörper (Klon 4B8) (ABIN2668226) detektiert spezifisch AGO1 in WB und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für AGO1 Antikörper (N-Term) (ABIN2668226)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser AGO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 4B8

Produktdetails anti-AGO1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Ago1 antibody (mAb) (Clone 4B8)

Aufreinigung: Protein G Chromatography

Immunogen: This Ago1 antibody was raised against a peptide from the N-terminus of human Argonaute 1 (Ago1) protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-AGO1 Antikörper (ABIN2668226)

Beitzinger, Peters, Zhu, Kremmer, Meister: "Identification of human microRNA targets from isolated argonaute protein complexes." in: RNA biology, Vol. 4, Issue 2, pp. 76-84, (2007) (PubMed).

Höck, Weinmann, Ender, Rüdel, Kremmer, Raabe, Urlaub, Meister: "Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells." in: EMBO reports, Vol. 8, Issue 11, pp. 1052-60, (2007) (PubMed).

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: Ago1

Hintergrund: The Argonaute family proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain.

Molekulargewicht: 100 kDa

Gen-ID: 26523

NCBI Accession: NP_036331

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization