PAX5 Antikörper (Alexa Fluor 647)

Produktnummer ABIN2667344

Dieses Anti-PAX5-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von PAX5 in FACS und BR. Geeignet für Human und Maus.

Kurzübersicht für PAX5 Antikörper (Alexa Fluor 647) (ABIN2667344)

Target: PAX5 (Paired Box 5 (PAX5))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser PAX5 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Flow Cytometry (FACS), Blocking Reagent (BR)

Klon: 1H9

Produktdetails anti-PAX5 Antikörper (Alexa Fluor 647)

Aufreinigung: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from prolonged exposure to light. Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody solution should be stored undiluted between 2°C and 8°C

Details zu PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Andere Bezeichnung: Pax-5

Hintergrund: Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (Pax) family of transcription factors. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. Pax5 is the only member of the Pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9,14)(p13,q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.