HMGB1 Antikörper

Produktnummer ABIN2666344

Produktdetails anti-HMGB1 Antikörper

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Intracellular Flow Cytometry (ICFC)

Aufreinigung: The antibody was purified by affinity chromatography.

Klon: 3E8

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Upon receipt, store undiluted between 2°C and 8°C.

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMGB1 (HMGB1 Produkte)

Synonyme: HMG1 antikoerper, HMG3 antikoerper, SBP-1 antikoerper, DEF antikoerper, HMG-1 antikoerper, Hmg1 antikoerper, amphoterin antikoerper, p30 antikoerper, hmgb1 antikoerper, ik:tdsubc_1a5 antikoerper, wu:fb23c02 antikoerper, xx:tdsubc_1a5 antikoerper, zgc:56110 antikoerper, zgc:77104 antikoerper, hmg-1 antikoerper, hmg3 antikoerper, sbp-1 antikoerper, hmg1 antikoerper, HMGB1 antikoerper, Ac2-008 antikoerper, high mobility group box 1 antikoerper, high-mobility group box 1 antikoerper, high mobility group box 1a antikoerper, high mobility group box 1 L homeolog antikoerper, high mobility group protein B1 antikoerper, HMGB1 antikoerper, Hmgb1 antikoerper, hmgb1 antikoerper, hmgb1a antikoerper, hmgb1.L antikoerper, LOC100359149 antikoerper

Hintergrund: High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse, rat, bovine and porcine HMGB1. HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome