HDAC10 Antikörper

Produktnummer ABIN265454

Der Kaninchen Polyklonal Anti-HDAC10-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, HDAC10 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für HDAC10 Antikörper (ABIN265454)

Target: HDAC10 (Histone Deacetylase 10 (HDAC10))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-HDAC10 Antikörper

Spezifität: This antibody detects endogenous levels of HDAC10 protein. (region surrounding Glu24)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5000-1/10000. Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HDAC10

Target: HDAC10 (Histone Deacetylase 10 (HDAC10))

Andere Bezeichnung: HDAC10

Hintergrund: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.Synonyms: HD10, Histone deacetylase 10

Molekulargewicht: approx. 75 kDa

Gen-ID: 83933

NCBI Accession: NP_001152758

UniProt: Q969S8