Filamin A Antikörper

Produktnummer ABIN265421

Der Kaninchen Polyklonal anti-Filamin A Antikörper wird verwendet zum Nachweis von Filamin A in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Filamin A Antikörper (ABIN265421)

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Filamin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Filamin A Antikörper

Spezifität: This antibody detects endogenous levels of Filamin A protein. (region surrounding Arg5146)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Filamin A

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Andere Bezeichnung: Filamin-A

Hintergrund: Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.Synonyms: Actin-binding protein 280, Alpha-filamin, Endothelial actin-binding protein, FLN, FLN1, FLNA, Filamin-1, Non-muscle filamin

Molekulargewicht: approx. 280 kDa

Gen-ID: 2316

NCBI Accession: NP_001104026

UniProt: P21333

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location