APAF1 Antikörper

Produktnummer ABIN265312

Produktdetails anti-APAF1 Antikörper

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects endogenous levels of APAF1 protein (region surrounding His324).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: APAF1 (APAF1 Produkte)

Synonyme: APAF1 antikoerper, ARK antikoerper, Apaf-1 antikoerper, Apaf1 antikoerper, CG6829 antikoerper, D-Apaf-1 antikoerper, DARK antikoerper, Dapaf-1 antikoerper, Dapaf-1/HAC-1 antikoerper, Dark antikoerper, Dark/Apaf-I antikoerper, Dark/Dapaf-1/HAC1 antikoerper, Dark/Hac-1/dApaf-1 antikoerper, Dark/Hac-1/dApaf1 antikoerper, Dmel\\CG6829 antikoerper, Hac-1 antikoerper, Hac-1/Dark antikoerper, Hac1 antikoerper, T1 antikoerper, anon-53Fa antikoerper, apaf-1 antikoerper, apaf1 antikoerper, arc antikoerper, ark antikoerper, dAPAF-1 antikoerper, dApaf-1 antikoerper, dApaf-1/DARK/HAC-1 antikoerper, dApaf1 antikoerper, dArk antikoerper, dapaf antikoerper, dapaf-1 antikoerper, dapaf-1L antikoerper, dapaf-1S antikoerper, dark antikoerper, dark/dapaf-1/hac-1 antikoerper, dark/hac-1/dapaf-1 antikoerper, hac-1 antikoerper, hac1 antikoerper, l(2)SH0173 antikoerper, APAF-1 antikoerper, CED4 antikoerper, 6230400I06Rik antikoerper, Apaf1l antikoerper, fog antikoerper, mKIAA0413 antikoerper, zgc:136543 antikoerper, Death-associated APAF1-related killer antikoerper, apoptotic peptidase activating factor 1 L homeolog antikoerper, apoptotic peptidase activating factor 1 antikoerper, Dark antikoerper, apaf1.L antikoerper, apaf1 antikoerper, APAF1 antikoerper, Apaf1 antikoerper

Hintergrund: Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein AFAP1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. AFAP1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413

Molekulargewicht: approx. 38 kDa

Gen-ID: 317

NCBI Accession: NP_001151

UniProt: O14727

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity