APAF1 Antikörper

Produktnummer ABIN265312

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch APAF1 in WB, IF, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für APAF1 Antikörper (ABIN265312)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-APAF1 Antikörper

Spezifität: This antibody detects endogenous levels of APAF1 protein (region surrounding His324).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: APAF1

Hintergrund: Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein AFAP1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. AFAP1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413

Molekulargewicht: approx. 38 kDa

Gen-ID: 317

NCBI Accession: NP_001151

UniProt: O14727

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity