SPARC Antikörper

Produktnummer ABIN265180

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch SPARC in WB, IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für SPARC Antikörper (ABIN265180)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-SPARC Antikörper

Spezifität: This antibody detects SPARC. It may Cross react with SPARCL1.

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from the Human SPARC protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: (1-5 μg/mL). ELISA: Immunohistochemistry on Frozen Sections. Positive Control/Tissue Expression: Mouse heart lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: SPARC / Osteonectin

Hintergrund: SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gen-ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagie