BrdU Antikörper

Produktnummer ABIN264907

Dieser Schaf Polyklonal Antikörper detektiert spezifisch BrdU in IHC (p), WB und IP.

Kurzübersicht für BrdU Antikörper (ABIN264907)

Target: BrdU (Bromodeoxyuridine (BrdU))

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-BrdU Antikörper

Aufreinigung: Protein G purified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 25 - 100 μg/mL. Immunoprecipitation: 25 - 100 μg/mL. Immunohistochemistry: 25 - 100 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, 0.08 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Substanzklasse: Chemical

Hintergrund: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.