SOD1 Antikörper

Produktnummer ABIN264856

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SOD1 in WB, IP, IF, IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schaf, Schwein, Affe, Hund, Hamster, Koralle und Xenopus laevis.

Kurzübersicht für SOD1 Antikörper (ABIN264856)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schaf, Schwein, Affe, Hund, Hamster, Koralle, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-SOD1 Antikörper

Spezifität: Detects 23 kDa (Human) and 19 kDa (Other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots.

Aufreinigung: Antigen Affinity Chromatography

Immunogen: Human Cu/Zn SOD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot (6,8): 0.2 μg/mL was sufficient for detection of Cu/Zn SOD in 20 g of HeLacell lysate. Immunoprecipitation (8). EIA (6). Immunohistochemistry on frozen sections(7).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS ( pH 7.0), 0.09 % Sodium Azide, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: Superoxide Dismutase 1 / SOD1

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

Gen-ID: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis