SOD1 Antikörper

Produktnummer ABIN264854

Produktdetails anti-SOD1 Antikörper

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: Detects a 23 kDa (Human) and 19 kDa (other species) proteins corresponding to the Molecular Mass of Cu/Zn Superoxide Dismutase (SOD) on SDS-PAGE Immunoblots.

Aufreinigung: Antigen Affinity Chromatography

Immunogen: Rat Cu/Zn SOD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5 μg/mL was sufficient for detection of Cu/Zn SOD in 20 μg of rat braintissue extract by Colorimetric Immunoblot analysis using Goat anti-RabbitIgG-AP as the secondary antibody. (Ref.6)Immunoprecipitation. EIA (Ref.6). Immunohistochemistry on Frozen Sections (Ref.8).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.0, 0.09 % Sodium Azide, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-SOD1 Antikörper (ABIN264854)

Zuber, Rappaport, Luo, Wang, Chen, Vaseva, Shi, Weissmueller, Fellmann, Fellman, Taylor, Weissenboeck, Graeber, Kogan, Vakoc, Lowe: "An integrated approach to dissecting oncogene addiction implicates a Myb-coordinated self-renewal program as essential for leukemia maintenance." in: Genes & development, Vol. 25, Issue 15, pp. 1628-40, (2011) (PubMed).

Wang, Kumagai-Braesch, Herrington, Larsson, Permert: "Increased lipid metabolism and cell turnover of MiaPaCa2 cells induced by high-fat diet in an orthotopic system." in: Metabolism: clinical and experimental, Vol. 58, Issue 8, pp. 1131-6, (2009) (PubMed).

Huang, Lee, Kuo, Yang, Chien: "Attenuation of long-term Rhodiola rosea supplementation on exhaustive swimming-evoked oxidative stress in the rat." in: The Chinese journal of physiology, Vol. 52, Issue 5, pp. 316-24, (2009) (PubMed).

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: Superoxide Dismutase 1 / SOD1 (SOD1 Produkte)

Synonyme: LOC692639 antikoerper, ALS antikoerper, ALS1 antikoerper, IPOA antikoerper, SOD antikoerper, hSod1 antikoerper, homodimer antikoerper, CG11793 antikoerper, Cu antikoerper, Cu-Zn SOD antikoerper, Cu/Zn SOD antikoerper, Cu/Zn sod antikoerper, Cu/Zn superoxide dismutase antikoerper, Cu/ZnSOD antikoerper, CuSOD antikoerper, CuZn SOD antikoerper, CuZn-SOD antikoerper, CuZn-SOD1 antikoerper, CuZnSOD antikoerper, Cu[2+]/Zn[2+]SOD antikoerper, Dmel\\CG11793 antikoerper, G antikoerper, Mn SOD antikoerper, SOD-1 antikoerper, SOD1 antikoerper, Sod-1 antikoerper, Sod1 antikoerper, To antikoerper, To-1 antikoerper, Zn SOD antikoerper, Zn Sod antikoerper, Zn-SOD antikoerper, ZnSod antikoerper, cSOD antikoerper, cSod antikoerper, dSOD1 antikoerper, l(3)108 antikoerper, l(3)68Af' antikoerper, l(3)G antikoerper, sod antikoerper, sod1 antikoerper, CU/ZN-SOD antikoerper, SODC antikoerper, DKFZP469M1833 antikoerper, B430204E11Rik antikoerper, Cu/Zn-SOD antikoerper, Ipo-1 antikoerper, Ipo1 antikoerper, SOD1L1 antikoerper, XSODB antikoerper, als antikoerper, als1 antikoerper, ipoa antikoerper, sod1-a antikoerper, ZSOD antikoerper, cuzn antikoerper, Cu/Zn superoxide dismutase antikoerper, superoxide dismutase 1 antikoerper, Superoxide dismutase 1 antikoerper, superoxide dismutase 1, soluble antikoerper, superoxide dismutase 1 S homeolog antikoerper, Superoxide dismutase [Cu-Zn] antikoerper, superoxide dismutase 1 L homeolog antikoerper, superoxide dismutase [Cu-Zn]-like antikoerper, superoxide dismutase [Cu-Zn] antikoerper, superoxide dismutase Sod1 antikoerper, SOD antikoerper, SOD1 antikoerper, Sod1 antikoerper, sod1 antikoerper, sod1.S antikoerper, sod-1 antikoerper, sod1.L antikoerper, LOC101451855 antikoerper, LOC101115136 antikoerper

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (Ref.3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (Ref.2,5). In general, SODs play a major role in antioxidant defense mechanisms. (Ref.4) There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (Ref.3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (Ref.7). SOD3 can also be distinguished by its heparinbinding capacity (Ref.1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

Gen-ID: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis