MITF Antikörper

Produktnummer ABIN264430

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MITF in WB, IP, IHC (fro) und EMSA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für MITF Antikörper (ABIN264430)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Electrophoretic Mobility-Shift Assay (EMSA)

Klon: C5

Produktdetails anti-MITF Antikörper

Spezifität: Recognizes in Western blotting a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor. This antibody does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.does not

Produktmerkmale: Synonyms: Microphthalmia-associated transcription factor, Mi-protein

Aufreinigung: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with anN-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. Genename: MITF

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Gel Supershift. Western Blot (1 μg/mL). Immunoprecipitation (2 μg/mg of protein lysate). Immunohistochemistry (Frozen and Paraffin Sections). Positive Control: 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclastcells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.08 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-MITF Antikörper (ABIN264430)

Gehlsen, Szaszák, Gebert, Koop, Hüttmann, Steven: "Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells." in: Journal of biophotonics, Vol. 8, Issue 6, pp. 466-79, (2016) (PubMed).

Hägglund, Berghard, Carlsson: "Canonical Wnt/?-catenin signalling is essential for optic cup formation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81158, (2013) (PubMed).

Yamada, Ohno, Aoki, Semi, Watanabe, Moritake, Shiozawa, Kunisada, Kobayashi, Toguchida, Shimizu, Hara, Yamada: "EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 600-10, (2013) (PubMed).

Nakano, Ando, Takata, Kawada, Muguruma, Sekiguchi, Saito, Yonemura, Eiraku, Sasai: "Self-formation of optic cups and storable stratified neural retina from human ESCs." in: Cell stem cell, Vol. 10, Issue 6, pp. 771-85, (2012) (PubMed).

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: MITF

Hintergrund: There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3Synonyms: Mi-protein, Microphthalmia-associated transcription factor

Molekulargewicht: 52-56 kDa

Gen-ID: 4286

UniProt: O75030

Pathways: Chromatin Binding