HLA-DR Antikörper (FITC)

Produktnummer ABIN264299

Der Maus Monoklonal anti-HLA-DR Antikörper wird verwendet zum Nachweis von HLA-DR in Proben von Human und Affe. Er wurde validiert für FACS.

Kurzübersicht für HLA-DR Antikörper (FITC) (ABIN264299)

Target: HLA-DR

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-DR Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: 423L

Produktdetails anti-HLA-DR Antikörper (FITC)

Produktmerkmale: Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Monitoring of B cells in peripheral blood. Analysis of B subsets. Study of B cell activation. Study of B cell neoplasms.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu HLA-DR

Target: HLA-DR

Andere Bezeichnung: HLA Class II DR

Hintergrund: Identification of human B cell and T cell subsets associated with approximately 10 % of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response