CD5 Antikörper (APC)
Kurzübersicht für CD5 Antikörper (APC) (ABIN264188)
Target
Alle CD5 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Produktmerkmale
- Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5
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Isotyp
- IgG2a
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Applikationshinweise
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Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Analysis of Bcell subsets, Characterization of subtypes of T cell leukemias and lymphomas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protokoll
- PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Buffer
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
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Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. -
Haltbarkeit
- 12 months
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- CD5
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Andere Bezeichnung
- CD5
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Hintergrund
- Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85 % peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
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Gen-ID
- 921
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UniProt
- P06127
Target
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