Der Maus Monoklonal anti-GAPDH Antikörper (Klon B903M) (ABIN263739) detektiert spezifisch GAPDH in WB, IF und EIA.
Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Kaninchen, Schwein, Rind (Kuh) und Ziege.
GAPDH
Reaktivität: Human
WB, ELISA, IHC, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA. Western Blot. Immunocytochemistry. Recommended pairs for Sandwich Immunoassay: Capture: ABIN263741Detection: ABIN263739 Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
4.5 mg/mL (Sigma protein assay kit)
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. Shelf life: One year from despatch.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36 kDa subunits.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase