Cathepsin K Antikörper (Internal Region)

Produktnummer ABIN263224

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Cathepsin K in WB, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für Cathepsin K Antikörper (Internal Region) (ABIN263224)

Target: Cathepsin K (CTSK)

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin K Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Cathepsin K Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Cathepsin K

Sequenz: KTHRKQYNNK VDE

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KTHRKQYNNKVDE, from the internal region (near N Terminus) of the protein sequence according to NP_000387.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 37 kDa band observed in Rat Skeletal Muscle lysates and in preliminary testing of Human Skeletal Muscle lysate (calculated MW of 36.8 kDa according to Rat NP_113748.1). Recommended concentration:0.1-1 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the vesicles of U2OSand MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Cathepsin K

Target: Cathepsin K (CTSK)

Andere Bezeichnung: CTSK

Hintergrund: CTSK, cathepsin K, CTS02, CTSO, CTSO1, CTSO2, MGC23107, PKND, PYCD, cathepsin K (pycnodysostosis), cathepsin O1, cathepsin O2, cathepsin X

Gen-ID: 1513

NCBI Accession: NP_000387

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades