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Vimentin Antikörper (C-Term)

Name: Vimentin Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Price: 779.48 EUR
Availability: InStock

Verified VIM Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated

ABIN2613493 (0. (Vimentin Antikörper (C-Term))

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin Antikörper (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin Antikörper (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin Antikörper (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin Antikörper (C-Term))

FACS

5 Abbildungen

Produktnummer ABIN2613493

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Bindungsspezifität
32

18

16

16

16

11

11

8

7

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reaktivität
323

125

122

60

58

43

42

36

30

22

20

14

13

8

7

6

6

3

3

2

1

1

1

1

1

1

1
Human, Maus, Ratte
Wirt
184

123

7

7

6

4

1
Ziege
Passende Sekundärantikörper
Klonalität
196

135
Polyklonal
Konjugat
188

19

14

13

10

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
271

127

96

96

92

56

43

40

39

28

23

20

8

5

4

4

4

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck

Vimentin

Sequenz

QVINETSQHH DDLE

Kreuzreaktivität

Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Isotyp

IgG

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VIM Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege WB, IHC, FACS, StM Wirt: Maus Monoclonal VM452 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 3A1F2 unconjugated

Applikationshinweise

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Vimentin (VIM)

Andere Bezeichnung

VIM (VIM Produkte)

Synonyme

CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper

Hintergrund

VIM, vimentin, FLJ36605

Gen-ID

7431, 22352, 81818

NCBI Accession

NP_003371

Pathways

Caspase Kaskade in der Apoptose