VCP Antikörper (Internal Region)

Produktnummer ABIN2613406

Dieses Anti-VCP-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von VCP in WB, IHC und ELISA. Geeignet für Human.

Kurzübersicht für VCP Antikörper (Internal Region) (ABIN2613406)

Target: VCP (Valosin Containing Protein (VCP))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VCP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-VCP Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CDC48 / YDL126C

Sequenz: HTKNMKLADD VDLE

Spezifität: This antibody may cross react in many other species.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte, Saccharomyces cerevisiae

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line HeLa (calculated MW of 92.0 kDa according to NP_010157.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu VCP

Target: VCP (Valosin Containing Protein (VCP))

Andere Bezeichnung: CDC48

Hintergrund: CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA

Gen-ID: 7415, 269523, 116643

NCBI Accession: NP_010157

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway