HSPB8 Antikörper (PerCP)

Produktnummer ABIN2486745

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HSPB8 in WB, IHC, IF, ICC und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für HSPB8 Antikörper (PerCP) (ABIN2486745)

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPB8 Antikörper ist konjugiert mit PerCP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-HSPB8 Antikörper (PerCP)

Spezifität: Detects ~22 kDa. Does not cross-react with HSP27 or alpha-crystallin.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Peptide Affinity Purified

Immunogen: Human HSP22

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN2486745 was sufficient for detection of HSP22 in 10 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu HSPB8

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Andere Bezeichnung: HSP22

Hintergrund: HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

Gen-ID: 26353

NCBI Accession: NP_055180

UniProt: Q9UJY1