CENPA Antikörper (Atto 390)

Produktnummer ABIN2486485

Der Maus Monoklonal Anti-CENPA-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, CENPA in Proben von Human zu detektieren.

Kurzübersicht für CENPA Antikörper (Atto 390) (ABIN2486485)

Target: CENPA (Centromere Protein A (CENPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CENPA Antikörper ist konjugiert mit Atto 390

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 5A7-2E11

Produktdetails anti-CENPA Antikörper (Atto 390)

Spezifität: Detects ~18 kDa. Recognizes Human CENP-A.

Kreuzreaktivität: Human

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide corresponding to a portion of human CENP-A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2486485 was sufficient for detection of CENPA in 20 μg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu CENPA

Target: CENPA (Centromere Protein A (CENPA))

Andere Bezeichnung: CENPA

Hintergrund: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Gen-ID: 1058

NCBI Accession: NP_001035891

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location