SCNN1A Antikörper (AA 46-68) (Atto 488)

Produktnummer ABIN2486334

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SCNN1A in WB, IHC, IF, IP und ICC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für SCNN1A Antikörper (AA 46-68) (Atto 488) (ABIN2486334)

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SCNN1A Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-SCNN1A Antikörper (Atto 488)

Bindungsspezifität: AA 46-68

Spezifität: Detects ~85 kDa.

Kreuzreaktivität: Human, Maus, Ratte, Xenopus laevis

Aufreinigung: Protein A Purified

Immunogen: Produced against a synthetic peptide mapping to the N-terminal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:400)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2486334 was sufficient for detection of alpha-ENaC in 35 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SCNN1A

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Andere Bezeichnung: ENaC

Hintergrund: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

Gen-ID: 25122

NCBI Accession: NP_113736

UniProt: Q6IRJ1