SLC12A1 Antikörper (AA 33-55) (Atto 594)

Produktnummer ABIN2486319

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SLC12A1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für SLC12A1 Antikörper (AA 33-55) (Atto 594) (ABIN2486319)

Target: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC12A1 Antikörper ist konjugiert mit Atto 594

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SLC12A1 Antikörper (Atto 594)

Bindungsspezifität: AA 33-55

Spezifität: Detects ~160 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2486319 was sufficient for detection of NKCC2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SLC12A1

Target: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Andere Bezeichnung: NKCC2

Hintergrund: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).

Gen-ID: 25065

NCBI Accession: NP_001257546

UniProt: Q5PQV1